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High sensitivity high efficiency high specificity ie a single peak in melting curve analysis and no primerdimers in the no-template control NTC. In Bio 6B youll use PCR in several different experiments.

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This page gives the background and protocol for one experiment while the other pages explain PCR in general and.

. The polymerase chain reaction or PCR is an incredibly useful and powerful technique for copying DNA. Both two-step and one-step RT-PCR are carried out. Successful amplification of the target is indicated by the following factors.

Background for all the PCR experiments. To find a QuantiTect Primer Assay for your target gene of interest please visit our GeneGlobe data base.

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